Genotypical and phenotypical characterization of Apilactobacillus kunkeei NFICC 2128 for high-mannitol scale-up production using apple pulp and sugar beet molasses as fermentable biological side streams

A combination of apple pulp (AP) and sugar beet molasses (M), two biological side streams, has been employed for high yield mannitol production. Apilactobacillus kunkeei is a heterofermentative obligate fructophilic lactic acid bacteria species (FLAB) which evolutively adapted to preferably metabolize fructose than glucose. A. NFICC 2128 strain, naturally isolated from Apis mellifera (honeybee), genotypical phenotypically studied in this project metabolism, production, optimization. Furthermore, mixture biowastes (2:1) used as fermentable substrates optimize upscaled productivity. Simultaneous fermentation enzymatic hydrolysis using invertase were needed achieve maximum yield. anaerobic, controlled pH (5.2) 30 °C conditions, among other tested at 1 L working volume, showed the highest 0.92 g / fructosei production 31.95 g/L mannitol.